Over pressure inAcquity UPLC BEH C18 column (1.7um, 2.1 x 100mm) column
We have been using an Acquity UPLC BEH C18 column (1.7 um; 2.1 x 100 mm) column for Mycotoxin analysis (DON) with a gradient from 10% methanol to 100% Methanol. recently we have see high back pressure with the column (over 14,000 psi). Purchased anew column and even just flushing with 10% Methanol see the same high back pressure. Any suggestions or solutions?