Over pressure inAcquity UPLC BEH C18 column (1.7um, 2.1 x 100mm) column

We have been using an Acquity UPLC BEH C18 column (1.7 um; 2.1 x 100 mm) column for Mycotoxin analysis (DON) with a gradient from 10% methanol to 100% Methanol. recently we have see high back pressure with the column (over 14,000 psi). Purchased anew column and even just flushing with 10% Methanol see the same high back pressure. Any suggestions or solutions?

Answers

  • Hi Alron1965,

    The M-Class backpressure limit is 15K - the 50/50 should not overpressure.  Is the pressure limit set correctly in the console and/or method editor?
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