How do I reduce noise in UHPLC MS data Peak Picking algorithm
Hi All
I am conducting an unsupervised analysis of a UHPLC MS data set in MarkerLynx. I am trying to improve the peak picking algorithm as my data noise is currently 70-85% when transferred into EZInfo Extended statistics package. I am sure this is due to the parameters of the algorithm but I have tried many many many ....many permutations of the various parameters. Is there a directed way to determine, by reading off the chromatograms the appropriate ranges to use for
spectral threshold, RT window, noise elimination window or any other of the method parameters available to clean up the peak picking process?
Thank you in advance
Christina
I am conducting an unsupervised analysis of a UHPLC MS data set in MarkerLynx. I am trying to improve the peak picking algorithm as my data noise is currently 70-85% when transferred into EZInfo Extended statistics package. I am sure this is due to the parameters of the algorithm but I have tried many many many ....many permutations of the various parameters. Is there a directed way to determine, by reading off the chromatograms the appropriate ranges to use for
spectral threshold, RT window, noise elimination window or any other of the method parameters available to clean up the peak picking process?
Thank you in advance
Christina
0
Answers
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Thank you for your question. Due to the technical nature of your question, please contact iRequest Technical Service/ Support: http://www.waters.com/waters/iRequest-Technical-Support/nav.htm?cid=10006027
A Waters Support Specialist will contact you to quickly address your needs. Thank you.
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