Dear All,Please kindly help me to choose the column for the polar compounds like (Metformin, Gemcitabine etc), I am using UPLC with PDA.Waiting for your feedback.You can also send me at [email protected] and regards,Aslam Burhan


  • Hello,

    The recommended reversed-phase UPLC column chemistry designed for the retention and separation of polar compounds is the ACQUITY UPLC HSS T3 chemistry.

    If the reversed-phase HSS T3 chemistry is not retentive enough, your next choice is to use hydrophilic interaction chromatography (HILIC). We have two HILIC UPLC phases: ACQUITY UPLC BEH Amide and ACQUITY UPLC BEH HILIC. The BEH Amide chemistry is a more universal HILIC phase whereas the BEH HILIC excels at retaining very polar basic compounds.

    Here is a LINK to the ACQUITY UPLC Columns brochure.

    If you have additional questions about ACQUITY UPLC Columns I suggest you contact yourt local Waters Chemistry Specialist. If you do not know who that is, please let me know and I will find that person's name for you.

    If you have any additional questions, please let us know.

    Good luck,


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