This is an area we are exploring, but no formal products as yet. We would require high pressure SPE and a way to multiplex to preserve the cycletime gains for UPLC. As we are looking into this area, can you share a little further information on what your applications is please.
Is anyone else looking at this too?
What chemistries would be necessary?
Many thanks for this interesting question.
Let me know any of the detail. Injection volume and application specifics - anything that would be necessary to add in to the ACQUITY system to ensure this worked.
Typical injection volume could be 2-4 ul. The application of on-line SPE is mostly for removing the interference and enriching the analyte concentration.
We are primarily looking at vitamin analysis from foods. The methods are LC-MS/MS and have the required selectivity but the sensitiivty and precision are boarderline in some instances. We would like to be able to achieve a 10X concentration of the analyte(s) using an on line SPE. Currently we are using 10 to 20 uL injections on either BEH C18 or HSS T3 columns.