Stable Isotope Internal Standards for LC-MS/MS
I have been doing LC-MS/MS method development for almost 15 years and have used stable isotope internal standards for much of that time. Recently I have been asked to start to tutor other staff in my group on quantitative LC-MS/MS method development. I have been trying to find references for the use of stable isotope internal standards but have been unsuccessful. Does anyone know where I can find references for a detailed explanation of the calculations, consideration and propagation of errors that occur with the use of stable isotope internal standards? Specifically I am looking for information regarding internal standard recovery limits and the propagation of error if the internal standard recovery is too low or too high, impact of density on the calculation (standards by volume but samples by weight), limitations for correcting matrix effects, and limits for using the internal standard to correct for sample dilution or concentration during sample preparation.