Degasser operating pressure range?

<p>Hello, I'm relatively new to UPLC. I've been using this system about 4 months now, and recently I'm having baseline drift problem that I had not seen before. I figured when I degassed the mobile phases manually, the baseline didn't drift. Now, the in-line degasser seems to be working okay. I mean if it's not, I would have gotten some sort of warning message?? We have 3 Acquities in our lab. The degasser pressure reading on each system is:</p><p></p><p>System #1: 0.42 psi</p><p>System #2: 0.49 psi</p><p>System #3: <strong><font color="#ff0000">0.68</font></strong> psi</p><p></p><p>The #3 is the one I've been using. Although not that big difference, I was wondering the somewhat higher pressure than other 2 is an indication of ineffective degassing? I've read thru the Operator's guide and the console help, but I couldn't find any statement about degasser. What is the proper operating pressure range? How would I know if the degasser is not operating properly? Waters team, please let me know... and fellow UPLC users, what is your system's pressure reading??</p><p></p><p>(My mobile phases are water and methanol with 5%IPA, running gradient, if that makes any difference with pressure reading) </p>

Answers

  • Hello:

    Do you have an example of the drift so that we can see it?

    I will get an answer on the values to expect for the pressure.

    Liz

  • Here you go.

  • Here is further information on ACQUITY degasser operation. The error message for the degasser will trip if the pressure goes above 1 psi for 30 seconds.

    Therefore the pressures that you are seeing are normal. The pressure differential are seeing is normal for system to system variation.

    The 0.2 difference he just the normal variance of the transducer.

    We do need to get more information about the problem to properly diagnose your issue.

    First can we get the raw data showing the issue with instrument conditions and method and to find out how you manually degassed the solvents and how you ran them without going through the degasser on the ACQUITY.

    Does the drift follow the gradient and does the drift go away when you stop flow? Letting the instrument set with flow going for a long time will also tell if the drifting is cyclic or random.

    Also can you include the detector conditions such as wavelength, lamp hours and lamp energy.

    This information should at least get us started in determining where to look.

  • This is helpful, but could we have the method info too, please!

    Many TX

    Liz

  • Thank you both for your reply. Unfortunately, I have to proceed with impending analyses, that I don't have time to investigate further. I know I can avoid this problem as long as I degas the mobile phases (with vacuum while stirring, 10 min), so I need to move on. I got the answer I wanted to know (the operating pressure range; it would be nice to have this basic information in the manual), I'm going to close out this thread. Thanks again!

  • One more thought....

    If the vent line from the degasser is restricted, the chamber pressure will increase. when the chamber pressure increases, the partial pressure of the remaining gases in the mobile phases increases. the only degasser chamber failure that i've seen in a BSM has been related to a poorly vented degasser outlet. the user should verify that the vent line is clear and unrestricted.

    if the chamber pressure increases, the dissolved air in the mobile phase will increase. what's interesting is that the event at 20 minutes into this gradient is much larger with the suspect chamber pressure. that event occurs when the organic (is it methanol?) is being jumped high - perhaps 100%. since i don't have all the conditions, this is all speculation but please check if

    1. the vent line isn't restricted (do not put it in solvent waste)

    2. the organic modifier isn't methanol

    3. the wavelength isn't below 260 nm (likely to be 220 - 230nm).

    Just in case!

    Liz

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