Carryover issue on UPLC
Dear All,Have you met carryover issue on UPLC system? Currently we are trying to develop an UPLC method for carbamazepine, and the carryover issue happened! The strong solvent and the weak solvent we used for the injection system are 100% acetonitrile and 10% methanol individually, the wash volumes for both solvents are 200 uL. We have tried to change the strong solvent to 100% methanol and to increase the wash volume to 600 uL but useless. We have met this similar issues on the other drug substances before. Since we could not overcome this issue on UPLC system, we back to HPLC system without any problems.Do you have any good suggestions to resolve this problem? Thanks a lot!