I believe this means unless you process the sample set the summary function custom fields will not calculate.
The trigger for the summary functions is the "summarize custom fields" function from within the sample set. Unless you process by sample set, this trigger will not be sent and therefore summary functions will not calculate.
Yes, you can do this using a Peak, Bool, Calculated custom field. Set your sample type to All to include both the standard and sample for consideration and set peak type to All. Search order is generally Result Set Only depending on your local SOP.
You need to label your specific reference solution when making the sample set for example Ref. Is it just 1 injection of this reference solution? If so, Then your formula becomes LT(Area,Ref.1.(Area)) and if you only need it for one particular peak you can set that peak as CCalRef1 in your processing method and the formula then becomes LT(CCalRef1[Area],Ref.1.(CCalRef1[Area]) and if you want to display only results for sample injections you can tag on at the end:
LT(CCalRef1[Area],Ref.1.(CCalRef1[Area]) +EQ(Sample Type,"Standard")*-1*50000 (make sure All or Nothing is set to Yes for this).
If its an average of the injections in the Ref solution the formula becomes
LT(Area,Ref.%..AVE(Area)) and add a Summarize Custom Fields as last line in Sample Set if so.
Leave the Use as to Position and your entries can be something like 0 = Outside of Optimum Area and 1 = Less than area of ref solution.
In sample set, add a "Clear Calibration" line before the Std1 line. Label the 6 injections of Std1 as S0101, label the 2 injections of Std2 as S0102. Label the Samples 1 and 2 as U1 U2 U3 and U4. After the std2 line, insert a Clear Calibration, then Calibrate (making sure the label Reference on the Calibrate line is set to S01* then Quantitate with the Label Reference on Quantitate set to U*). In the component editor of your sample set make sure you have the amounts or concentrations entered in the Value tab for all standard components. Save all changes, bring the standard/sample channels into review, open processing method, and for the standard injections press integrate then calibrate, which builds your calibration curve. File>Save as Calibration to save curves. Then for your sample injections press integrate then quantitate and your amounts will appear.
Or you can batch process a sample set to generate a result set. Right click sample set, process, use specified processing method, select the method, make sure clear calibration is ticked then click ok- your result set will be generated with amounts stored. Make sure to account for any dilution factors or sampleweights for your samples before saving sample set.
Use same labels for injections of interest and process .
Then in report use table properties and summary functions (see attached)
Or Create a custom field but a bit pointless with report option