Acquity sample loop volume specifications?
<p><span>Could someone at Waters post the specifications, or range of volumes that one can expect to encounter with various sample loops?</span></p><p></p><p><span>The reason I ask is that my 2-microliter loop measured 3.0-uL last week when characterized on the Acquity that I have been using for development work.</span></p><p></p><p><span>If I develop and validate a method using "full loop" mode, and then need to transfer that method to a different lab that has an Acquity where the loop may be 1.8-uL, then the difference between peak heights of the two systems may vary as much as 40% from the original 3-uL volume. This could be sufficient to create accuracy problems with the data, despite a "validated" method being used. Full loop mode is desired for validation activities due to the need to maintain low RSD of injection volumes for an assay validated to comply with my employers validation SOPs.</span></p><p></p><p><span>If I know the range limits or each respective loop size, then this potential for variation could be designed into experiments for the loop size selected for the assay method. </span></p>