Running lots of standards in one run, best Fit Type
I'm running about 15-20 standards in a run, injected after every 6 samples, and I want to change the set up to modified standard throughout so inject a standard every 6 samples, add that point to the calibration curve and at the end ill have up to 20 standard points in the cal curve and use this curve to quantify ALL the previous samples. Is this more accurate than running sliding with/without overlap? I would have though using all the standards would be more accurate but another argument is that retention time and viscosity shifts may require the overlap method. What fit type is best for this- Linear thru Zero?
Also, whats the difference between Average by Amount and Average by Level? If you run 20 standards that all have the same concentration (eg 10.007 mcg/mL) and you put this in the Value of your component editor surely average by amount will average ALL these standards, same as if you set a Level of 1 next to these standards also?
Thanks for reading.