Which is better ACQUITY UPLC H-Class PLUS Bio System or ACQUITY UPLC I-Class PLUS

Mainly purity/aggregation analysis of purified proteins by SEC


  • per_oxid
    edited November 2021
    I-class is low dispersion and can handle microbore columns 1mm if you minimize all dwell volumes and use fixed loop, also come in a  flow through needle injector version. H-class has slightly higer dispersion can handle higher flow (2.2ml/min vs 2.0ml/min) probably litte more robust due to larger cappilaries. I assume you look at the binary pump versions.

    The max peak or premiere version can bring considerable improvements for som analytes. I am also interested in what the difference are between bio version and premiere versions in in terms of sample and solvent compatability.
  • Thank you so much for the answer.

  • Is the Auto•Blend Plus Technology available in the H- Class and the I class