3 mL injection volume - is that possible?

Juwe
Juwe
in Instrumentation and Mass Spectrometry
Hi all,
I have found a paper online in which a UPLC is described. It is stated that the method was perfomed using a Acquity UPLC from Waters. The indicated injection volume is 3 mL. Is that possible? Is there such a big loop?
Thanks in advance!

Answers

  • MarcNoble
    MarcNoble

    Hi Juwe,

    There is no sample loop for the ACQUITY UPLC that large, the largest injection that can be made on an ACQUITY UPLC with fixed loop injector is 250uL.  The Flow Through needle designs can inject up to 1mL.

    Sorry I couldn't be of more help on this.

    Marc

  • Juwe
    Juwe
    Hey Marc,

    Thanks for you answer. In the meantime I did some research and found out that it must have been a typing error in the paper. The injection should be perfomed with 3 µL. This definitely makes more sense.

    Juwe

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