Disturbing peak H-Class
I have a prblem with our H-Class. For qualification purpose, I inject a caffeine solution. The eluent is MeOH/water 20/80, isocratic. My caffeine peak elutes at approx. 1 min. Unfortunately there is a huge solvent/system "peak" which elutes from approx. 0.6 min to 1.2 min.
We changed all reagents (several times) already and tried different grades of them. Additionally we filtered the eluent. But the "peak" is still there. Please see the attached file.
Has anybody an idea what we could do to eliminate the "peak"?
Thanks in advance!