Missing Peaks Issue when Batch Processing in Empower 3
Hello,
I am seeing a strange issue when I am batch-processing samples in Empower 3. I am using an ApexTrack processing method (let's call it X). When I run a sample set and process it using a method set (i.e. run sample, process using X, and generate a PDF report), I see the integration of peaks as expected (for example, let's say for an SEC chromatogram, you see a small aggregate peak and the main species peak running as expected, with 1% aggregate peak and 99% main peak). However, there are also a few other 'peaks' that show up. The 'Int Type' column says 'Missing'. They are at random retention times, but none of these peaks are part of the integrated chromatogram and add nothing to the peak area. The PDF report just includes these missing peak retention times as lines on the table. I get the same behavior if I batch process samples after a run in the project window (i.e. right-click-->Process).
However, when I integrate any of these samples individually using the exact same processing method X, these 'missing' peak entries disappear from the table and the integration is still the same as before (1% aggregate peak and 99% main peak). I contacted the Waters phone support and the suggested approach was that perhaps if the sample set was getting interrupted, this may be causing this type of glitch, but running a fresh sample set without interruption does not resolve the issue.
Does anyone have thoughts on what may be causing this behavior and how to address it?
This is a screenshot of the issue. When batch-processing, you see the 'Missing' lines 4-6 in the table. When you process individually, these lines disappear.
I am seeing a strange issue when I am batch-processing samples in Empower 3. I am using an ApexTrack processing method (let's call it X). When I run a sample set and process it using a method set (i.e. run sample, process using X, and generate a PDF report), I see the integration of peaks as expected (for example, let's say for an SEC chromatogram, you see a small aggregate peak and the main species peak running as expected, with 1% aggregate peak and 99% main peak). However, there are also a few other 'peaks' that show up. The 'Int Type' column says 'Missing'. They are at random retention times, but none of these peaks are part of the integrated chromatogram and add nothing to the peak area. The PDF report just includes these missing peak retention times as lines on the table. I get the same behavior if I batch process samples after a run in the project window (i.e. right-click-->Process).
However, when I integrate any of these samples individually using the exact same processing method X, these 'missing' peak entries disappear from the table and the integration is still the same as before (1% aggregate peak and 99% main peak). I contacted the Waters phone support and the suggested approach was that perhaps if the sample set was getting interrupted, this may be causing this type of glitch, but running a fresh sample set without interruption does not resolve the issue.
Does anyone have thoughts on what may be causing this behavior and how to address it?
This is a screenshot of the issue. When batch-processing, you see the 'Missing' lines 4-6 in the table. When you process individually, these lines disappear.
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Comments
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When your processing method contains named peaks and the chromatograms do not contain them (or the retention windows do not contain the apexes of peaks), you get Missing results. This is normal.0
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Thanks for the reply! Hmm...is there a reason why I would get missing results when bulk processing and the missing results disappear when I individually process with the same process method then?Dan_Ramlose said:When your processing method contains named peaks and the chromatograms do not contain them (or the retention windows do not contain the apexes of peaks), you get Missing results. This is normal.0 -
Ah, never mind, it works now. Thanks a lot!0
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So, what change fixed it?0
