Waters 2695/PDA 2998 baseline issues
So I’m kinda new to HPLC. I am using Waters 2695 equipped with PDA 2998. I have several issues with / questions about the equipment:
1. When I tried to make the calibration curve of atrazine, I got a noisy/drafty baseline. I’ve attached images to this post. Any ideas of how to fix that?
2. When I tried to make the calibration curve of 2,4-D at different concentrations, there were no peaks at some concentrations! Any thoughts?
3. What is the correct procedure for turning off the equipment? Just to flush the column with the more organic solvent and then turning it off?
I appreciate your responses.