Bracketing standards in sample set
Im looking to inject samples/standards one after the other and according to Empower Help, the best way to do that in the sample set is:
Clear Calibration
Inject Standards
Inject Samples
Clear Calibration
Inject Standards
Inject Samples
Clear Calibration
etc etc
Processing of all lines is set to Normal.
How does Empower know to quantitate that one sample by the preceding standard if nothing is labelled or no quantitate lines are specified? Im used to setting up my sample set with bracketing standards before and after 10 sets of samples then calibrate with my standard labels and quantitiate with the unknown (U01* etc) labels but the above set up seems very basic.
Also, does it matter if you inject samples first then standards? Will Empower still quantify all peaks in the sample off the following standard?
Clear Calibration
Inject Samples
Inject Standards
Clear Calibration
Inject Samples
Inject Standards
Clear Calibration
etc etc
Clear Calibration
Inject Standards
Inject Samples
Clear Calibration
Inject Standards
Inject Samples
Clear Calibration
etc etc
Processing of all lines is set to Normal.
How does Empower know to quantitate that one sample by the preceding standard if nothing is labelled or no quantitate lines are specified? Im used to setting up my sample set with bracketing standards before and after 10 sets of samples then calibrate with my standard labels and quantitiate with the unknown (U01* etc) labels but the above set up seems very basic.
Also, does it matter if you inject samples first then standards? Will Empower still quantify all peaks in the sample off the following standard?
Clear Calibration
Inject Samples
Inject Standards
Clear Calibration
Inject Samples
Inject Standards
Clear Calibration
etc etc
0
Best Answer
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When the processing column is set to normal, Empower automatically generates a cal curve on the std, then use that active cal curve on subsequent injections until cleared. It simply goes row by row, no jumping around in the sample set, no looking below the current row during processing, and everything is processed in the order it is listed in the sample set/order it was acquired.
The calibrate/quantitate instructions allow you to tell Empower to process things in an order that is not consistent with how the data was acquired which is where you can use a std before/after some samples to generate a cal curve which can then be used to quantitate sample between them. If you are jumping around like that, then you'd use the do not process instruction in the processing column for the injections to have Empower skip the row until your calibrate/quantitate instruction tells it to go there.
So if you were to swap the order, you'd get your chromatograms and areas, but the samples would not have any amounts as the clear calibration removes any cal curve. Then you'd effectively generate a useless calibration curve as it gets cleared on the next row.
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