How Does Empower 2 Calculate Total Sample Set Time?
<p>While I was preparing to explain to some users how Empower 2 (SP G, HF 2) will calculate the total time required for their runs.
I noticed that it tacked an additional 1 min 21 sec per injection beyond the run time, and assumed it was taking into account the delay between injections.
However, I cannot determine where this number came from. I checked what it is on a variety of different instruments. Here is what I got:
.</p><table border="1" cellpadding="3" cellspacing="0" style="width: 100%; border: #000000 1px solid;"><tbody><tr><td align="center" colspan="5" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>Added Time (sec)</strong></span></td></tr><tr><th align="center" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>H-Class UPLC</strong></span></th><th align="center" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>Alliance</strong></span></th><th align="center" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>Agilent 1100</strong></span></th><th align="center" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>Agilent 1200</strong></span></th><th align="center" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>Agilent 7890</strong></span></th></tr><tr><td style="text-align: center;">34</td><td style="text-align: center;">55</td><td style="text-align: center;">81</td><td style="text-align: center;">66</td><td style="text-align: center;">147</td></tr><tr><td style="text-align: center;">0</td><td style="text-align: center;">0</td><td style="text-align: center;">68</td><td style="text-align: center;">0</td><td style="text-align: center;">550</td></tr><tr><td style="text-align: center;">31</td><td style="text-align: center;"></td><td style="text-align: center;"></td><td style="text-align: center;"></td><td style="text-align: center;"></td></tr><tr><td style="text-align: center;">7</td><td></td><td></td><td></td><td></td></tr></tbody></table><p>
So I'm assuming Empower isn't making these values up, but have no idea where they came from. Any input would be appreciated!</p>
I noticed that it tacked an additional 1 min 21 sec per injection beyond the run time, and assumed it was taking into account the delay between injections.
However, I cannot determine where this number came from. I checked what it is on a variety of different instruments. Here is what I got:
.</p><table border="1" cellpadding="3" cellspacing="0" style="width: 100%; border: #000000 1px solid;"><tbody><tr><td align="center" colspan="5" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>Added Time (sec)</strong></span></td></tr><tr><th align="center" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>H-Class UPLC</strong></span></th><th align="center" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>Alliance</strong></span></th><th align="center" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>Agilent 1100</strong></span></th><th align="center" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>Agilent 1200</strong></span></th><th align="center" style="background-color:#6690BC;" valign="middle"><span style="color: #ffffff;"><strong>Agilent 7890</strong></span></th></tr><tr><td style="text-align: center;">34</td><td style="text-align: center;">55</td><td style="text-align: center;">81</td><td style="text-align: center;">66</td><td style="text-align: center;">147</td></tr><tr><td style="text-align: center;">0</td><td style="text-align: center;">0</td><td style="text-align: center;">68</td><td style="text-align: center;">0</td><td style="text-align: center;">550</td></tr><tr><td style="text-align: center;">31</td><td style="text-align: center;"></td><td style="text-align: center;"></td><td style="text-align: center;"></td><td style="text-align: center;"></td></tr><tr><td style="text-align: center;">7</td><td></td><td></td><td></td><td></td></tr></tbody></table><p>
So I'm assuming Empower isn't making these values up, but have no idea where they came from. Any input would be appreciated!</p>
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Answers
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Hi smart ,
It may be the time to take for an injection.
Agilent: If you enable needle wash in Agilent.
Waters: Needle wash+Injection time.
If i am wrong let me know please.
Regards
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Clearly, though, 0 or 7 seconds is way too small for any of that, and almost 10 min is way too long. As I said, I originally thought that as well, but that is clearly not the case for some of these.
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Why cont we go through Instrument settings.
I am not sure but we can find something.
Regards
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