Change in retention time between Acquity Binary system and H-Class
I have been working to show some robustness of a method as it transfers from our original Acquity binary based system with a PDA to a H-Class quaternary based system with a TUV. The baseline and responses are good, but I am getting a strong shift in retention time as I move to the H-Class (approx 1 minute for everything). The void volume seems to be the same as the non-retained compounds all elute at 0.4 min for both systems, and I'm having some trouble explaining this shift. Any help would be appreciated, thanks.