signal to noise by using blank injection

ra493862
ra493862
in Software Products
<p> how can i do it?</p><p>please look at the attached</p>

Answers

  • rune
    rune

    Hi Raffael

    If you are using Empower3 you do not need a custom field to do this calculation. It is part of the system suitability calculation options.

    See attachement on how to set this up.

    best regards

    Rune

  • ra493862
    ra493862

    dear rune

    thanks for your answer

    please see the attached

    thanks

    raffael

  • rune
    rune

    Dear Raffael

    What I forgot to mention was that you need to specify which injection is the blank. This is done in the sampleset where you have a new column called blank. If you specify more than one blank injection Empower will use the average noise values from the blank injections. Remember that the blank injections must come before the samples and standards in the sampleset.

    So step by step here is what you need to do :

    1. Mark the blank injections in the sample set.

    2. Create the processing method with the system suitability set to :

    •     Calculate USP, EP, JP s/n
    •     Use noise centered on the peak region in blank injection
    •     Specify what noise value for s/n you will use (I recommend peak to peak noise)

    3. Specify that you want to calculate detector noise and drift. It does not matter what noise interval you choose, since Empower will calculate the noise for each peak from the blank injection in the region where the peak elutes.

    4. Process you sampleset.

    Empower will then calculate the signal to noise ratio s/n using the noise from the blank injection but the signal (peak height) from the actual chromatogram. It will calculate the s/n for every integrated peak in the chromatogram.

    I have update the presentation to reflect what you need to do.

    I hope this helps.

    Best regards

    Rune

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