Best way to equilibrate HSS T3?
Just curious about opinions regarding best way to equilibrate an HSS T3 column. I know it's 100% aqueous compatible, but is it better to have a touch of organic in there? Which one (ACN, MeOH...)? I run at 40oC. Reason I ask is because I feel like I may have "lost" an analyte, in other words I had 20 good runs in a row where I saw two analytes, now I am only seeing one. Did my column give up the ghost or does it just need a stern talking to, i.e. perhaps a good long cleaning (in what?) or just a good long equilibration (in what?). Any other wisdom to help me find my lost analyte? Thanks!