Best injection mode to prevent carryover?
I have an assay that frequently exhibits carryover, and is very sensitive to the concentrations of formic acid in my mobile phases and needle washes. Between the full loop and partial loop injection mode, which is better to prevent carryover? I currently run a 5 µL injection on a 10 µL loop and sample consumption is not really an issue. More specifically, of these two types of injection modes, will one type expose the actual sample to more needle wash, or will one type wash my injection port area more thoroughly?Thank you,Laura