Anyone ever used BEH-Amide for NDP-Sugars?
Hope someone can help me out. I have a user who is trying to finish up his degree and leave, but needs me to do LCMS on some NDP-Sugars he has synthesized. He doesn't have time to purify them and his LC method isn't MS compatible. I'm trying to use a BEH-Amide column, but don't have much experience with this chemistry. I've been using a gradient method (5 min 85-70%B, hold @ 70 for 5 min then back to 85 and hold) using acetonitrile for B and 5 mM ammonium acetate for A. If I do a long pre-equilibration I get retention, but in subsequent injections I don't get retention. I'm hoping someone has some tips to help me out.Thanks,Cameron