Is this BSM plot normal?
Hi, AllI am working on UPLC-MS analysis and noticed the MS signal intensity dropped abruptly and significantly (100 times lower) during the gradient HPLC analysis. I did all the trouble shooting and is confident that MS is working well.This leads me to believe that the UPLC is not pumping as it should be during the gradient. (I also tried isocratic at 2%B, the MS intensity remains constant). I had difficulty to attach the BSM plot here, but I enclosed the figure in the ppt. Can anyone help me to take a look? This is 2% B at 50uL/min.Dongdong