Is this BSM plot normal?
<p> Hi, All</p><p></p><p>I am working on UPLC-MS analysis and noticed the MS signal intensity dropped abruptly and significantly (100 times lower) during the gradient HPLC analysis. I did all the trouble shooting and is confident that MS is working well.</p><p></p><p>This leads me to believe that the UPLC is not pumping as it should be during the gradient. (I also tried isocratic at 2%B, the MS intensity remains constant). I had difficulty to attach the BSM plot here, but I enclosed the figure in the ppt. Can anyone help me to take a look? This is 2% B at 50uL/min.</p><p></p><p>Dongdong</p>