loop offline or online?
<p>Hello,</p><p></p><p>I have a few questions regarding the way the Acquity injector works.</p><p></p><p>1. During normal injection mode (no load ahead), does the mobile phase flow through the injection loop during the whole run or does it go to offline (load) position after a certain time? I think it stays online during the whole run but I am not entirely sure.</p><p></p><p>2. In load ahead mode, when working in partial or full loop, one needs to set a time after which the injector gets offline. I understand this time should be long enough to enable high organic solvent to go through the loop. However I guess it should be short enough to enable the next injection load to occur within the run. So my question is: how much time before the next injection should the injector be set offline?</p><p></p><p>3.In partial loop with needle overfill mode, one doesn't need to set this offline value. Do you know why? Does it mean that in PLWNO mode, the injector automatically switch to offline position after a certain time?</p><p></p><p>Thanks for your reply.</p>