Size Exclusion on the UPLC
We've been using a Size Exclusion column on our HPLC which runs with isocratic 50% ACN with 0.1% TFA to analyze wheat proteins. The Waters Size Exclusion columns for the UPLC use a maximum of 20% organic. We're concerned that our proteins will come out of solution with only 20% organic but we would really like to try the UPLC. Given the cost of the columns we are reluctant to order one just to possibly have it fail. Has anyone transferred a HPLC-SE method to the UPLC? If so, what are your conditions? Any help would be appreciated.