General failure
Greetings ,Did anyone had this error message for his BSM in UPLC system : General failure ?Do you know what this means ?where can i find any file that contains every possible error message in UPLC system and its explanation ?Best regards ,M.K.
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Answers
Hello,
We have seen this several times and think that it comes from having small time gaps in your gradient method, for example:
0.00 10%
1.00 10%
1.05 90%
The gap of 0.05seconds and the jump from 10 - 90% seems too much for the pumps to cope with. We found that if you increase the step time to 0.1 or 0.2 then these problems go away.
David
To have proper step gradient you could use curve type 1 or 11.