General failure

Greetings ,Did anyone had this error message for his BSM in UPLC system : General failure ?Do you know what this means ?where can i find  any file that contains every possible error message in UPLC system and its explanation ?Best regards ,M.K.

Answers

  • Hello,

    We have seen this several times and think that it comes from having small time gaps in your gradient method, for example:

    0.00  10%

    1.00  10%

    1.05  90%

    The gap of 0.05seconds and the jump from 10 - 90% seems too much for the pumps to cope with. We found that if you increase the step time to 0.1 or 0.2 then these problems go away.

    David

  • To have proper step gradient you could use curve type 1 or 11.

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