Acquity Sample Manager Advanced Settings
I am currently making a 0.2 uL injection using a 2 uL injection loop in PLNO injection mode. Load ahead is enabled. Loop offline disabled. Refer to screen dump in attached doc.Mobile phase A1 is 9%v/v MeOH in Ammonium formate pH 9.6Mobile phase B2 is 9%v/v Ammonium formate in MeOH pH 9.6When I inject a standard containing morphine, codeine, oripavine and thebaine in 1% v/v acetic acid in MilliQ Water, the normalised area for each compound is lower than the normalised peak areas for the same compounds dissolved in a diluent composed of 30:10:70 - Ethanol:Acetic Acid:MilliQ Water.Are there any advanced settings I can alter in my instrument method to reduce the differences?Is a change to Full Loop overfill factor applicable?Are air gaps set ok?Should I increase needle overfill flush and how would this help?Are the differences likely due to changes in sample viscosity or adsorption within the needle/sample loop?