UPLC for SDS/BME containing protein sample
I am currently trying to transfer an old HPLC method from another group that uses approximately 2% SDS and 5% BME. The old method uses a Bakerbond C8 column, 5 µ, 300A, 4.6 ´ 100 mm (Baker Cat. #7105-01) and I would like to use a UPLC column instead. The column does not have to match this one exactly as far as ligand, but the 300A pore size is a must as this is a big protein.Any suggestions that anyone can offer would be great. I have an Acquity BEH300 C4 column that I am tempted to try this on but am afraid of destroying the column.