Fast(er) SEC- is there a way to stagger injections and data collection?
<p>We have been looking at ways to speed up our size exclusion assays, for faster throughput for screening of formulation samples. We have looked at the Acquity BEH SEC column, and found it to work great - immediately cut our run times by at least half, and possibly more as we work to optimize the method. </p><p></p><p>Looking at what else we can do, I recently came across an article that made a valid point- for an SEC column, all of the separation comes between the exclusion limits and the inclusion limits of the column, so it is really unnecessary to collect data until you reach the exclusion limit. Their idea was to stagger injections; for example, for a conventional column, you would inject, wait ~10 minutes (depending on the properties of your column), and then while you are acquiring data for the first sample where the sample actually separates, inject the sample again, so that you are constantly collecting data over the ~15 minutes or so where the separation is actually happening (I hope that makes sense). This would significantly cut run times, and would be really great for rapid screening.</p><p></p><p>Thinking about the options on the UPLC, though, I'm not sure if it would be possible to run the instrument this way. I'm hoping a more expert user might weigh in as to how it may be done.</p><p></p><p>The paper I was looking at is: J Chromatog A 1216 (2009) 8904-8909.</p><p></p><p>Thanks!</p>