Integration parameters in masslynx and targetlyx
Answers

Smoothing
Smoothing
I have inserted some text from the MassLynx help files for reference:
Two types of smoothing are available for chromatograms; Moving Mean and Savitzky Golay. Both methods slide a window along the chromatogram, averaging the data points in the window to produce a point in the smoothed spectrum. Moving Mean takes the arithmetical mean of the intensities of the data points in the window. Savitzky Golay takes an average of the intensities weighted by a quadratic curve. This tends to enhance peak and valley shapes, as well as preserving the height of the peaks better than the Moving Mean. However, Savitzky Golay does tend to produce small artifacts on either side of the real peaks.
Once the parameters have been selected, click OK to return to the Integrate chromatogram dialog.
I have attached a files showing a peak smoothed using mean (2x3) and SG (2x3) The SG smoothed peak is a similar intensity to the unsmoothed peak but there are artefacts on the peak, this may cause some issues when integrating the peaks. The mean smooth is a wider peak but it will provide a much more consistent integration. I have chosed parameters that illustrate the point. As a rule of thumb, I use a mean smooth for chromatography choosing parameters that do not distort the peak. In this case I would use a 2x1 mean smooth.
Regards,
Jody Dunstan.
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Thanks Jody,
I have asked so many people this question and no one seemed to know the answer. Much appreciate your reply.
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