How to determine if peek elutes in dead time - ACQUITY UPLC-MS/MS System - minumum RT of peek?
I see in the forum there is a question asking what is the minimum gradient analysis time you have used with your acquity UPLC system and one of the options is 0.5 min. I would have thought that analytes would elute in deadtime if your run was this short (when using MS as detector is there any practical way of knowing the dead point other than using a theoretical calculation)? I thought anything less than about 0.75 mins elutes in dead time (dependant on column used and flow rate). I have developed a method and put a hold of 0.5 mins and then start the gradient slowly to hold the compounds for nearly a minute so prevent the analytes eluting in dead time. What is the earliest time compounds can reliably elute? Is it different for MS detectors compared to for example a UV detector as with LC-UV you can see the solvent front and know if your peek elutes before/after/with this??