I am having problems maximizing the recovery from maximum recovery vials. My reference material is severely limited and not being able to recover everything from the vials is crippling my method development. I was told to use maximum recovery vials as opposed to total recovery vials due to the way the needle samples from the vials. And I have changed the z-axis of the needle (although I don't know how to tell what the it is at currently???). I am scheduled to take the UPLC training course in May, but my questions can not wait until then.Any suggestions????