Coupling UPLC columns in series

RobBurgess
RobBurgess
in Instrumentation and Mass Spectrometry
<p><span>What are people using to say couple 2 x 15 cm columns for high resolution applications. Is there any way to enable as low to zero volume connection as possible? Also how would you fit into the column oven?</span></p>

Comments

  • dougm
    dougm

    Hello,

    I checked with a few of our scientists and they all stated pretty much the same thing. Connect the columns with either a 1uL (430001516) or 2uL (430001264) loop as the connecting tube(s). Work involving connecting UPLC columns in series at Waters used the Column Manager which enables you to easily use multiple columns. However, if you have the Column Oven, it is much more difficult (challenging) since the Column Oven was designed to accomodate one column only.

    I hope this helps.

    --Doug

  • Nicki-Nitro
    Nicki-Nitro

    Hi Rob,

    Did you have much success?

    Regards

    Ronald

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