A novel reversed-phase and HILIC method development strategy on the new ACQUITY UPLC H-Class

<p>The retention and quantitation of polar analytes and metabolites continues to be an exceptional chromatographic challenge for analytical scientists. Reversed-phase is the most universally accepted and implemented technique, however, it is not particularly well suited for analytes that are hydrophilic [polar]. </p><p></p><p>Hydrophilic interaction chromatography [HILIC] is a complementary chromatographic technique that can be used to successfully improve the retention of very polar species. However, due to the intricate combination of multiple retention mechanisms, HILIC is also a useful technique for the retention and improved MS response of ionizable species, regardless of their polarity. </p><p></p><p>The new capabilities of the ACQUITY UPLC H-Class System enable the sequential development of methods using a novel reversed-phase and HILIC approach. This innovative approach provides a comprehensive combination of selectivity choices to effortlessly retain and resolve compounds encompassing a broad range of polarity. </p><p></p><p></p><p>Learn more about the industry-leading collection of UPLC column chemistries and novel chromatographic strategies by clicking on the link below:</p><p></p><p><a href="http://www.waters.com/waters/nav.htm?cid=513206">http://www.waters.com/waters/nav.htm?cid=513206</a></p><p></p><p>Not sure about what HILIC is or how to be successful with it? Learn more with this interactive web tool:</p><p></p><p><a href="http://www.waters.com/hilic">http://www.waters.com/hilic</a></p>