Chiral UPLC

<p><span>Hello,</span></p><p><span>Just wondering if anyone is doing chiral UPLC using achiral UPLC columns and chiral additives in mobile phase. Any suggestions/information will be much appreciated.</span></p>


  • dougm


    I was hoping that a UPLC user would reply to your request since Waters presently does not have direct experience in this area. We are aware of publications in this area. I've attached a couple references for your review.

    This publication will likely be available in hard copy later this year.

    Chirality. 2009 Jun 18. [Epub ahead of print]

    Fast chiral separation of drugs using columns packed with sub-2 um particles and ultra-high pressure.

    Guillarme D, Bonvin G, Badoud F, Schappler J, Rudaz S, Veuthey JL.

    School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, 1211 Geneva 4, Switzerland.

    The use of columns packed with sub-2 um particles in liquid chromatography with very high pressure conditions (known as UHPLC) was investigated for the fast enantioseparation of drugs. Two different procedures were evaluated and compared using amphetamine derivatives and beta-blockers as model compounds. In one case, cyclodextrins (CD) were directly added to the mobile phase and chiral separations were carried out in less than 5 min. However, this strategy suffered from several drawbacks linked to column lifetime and low chromatographic efficiencies. In the other case, the analysis of enantiomers was carried out after a derivatization procedure using two different reagents, 2,3,4-tri-O-acetyl-alpha-D-arabinopyranosyl isothiocyanate (AITC) and N-alpha-(2,4-dinitro-5-fluorophenyl)-L-alaninamide (Marfey's reagent). Separation of several amphetamine derivatives contained within the same sample was achieved in 2-5 min with high efficiency and selectivity. The proposed approach was also successfully applied to the enantiomeric purity determination of (+)-(S)-amphetamine and (+)-(S)-methamphetamine. Similar results were obtained with beta-blockers, and the separation of 10 enantiomers was carried out in less than 3 min, whereas the individual separation of several beta-blocker enantiomers was performed in 1 min or less.

    Chirality, 2009. (c) 2009 Wiley-Liss, Inc.

    Here is a link to an abstract posted by some researchers from Novartis (I've not seen the talk/poster/publication):

    Good luck,