2ul loop calibration
I am looking for some information with regards to chracterization of a 2ul loop. Without exception everytime I place a 2ul loop on my Acquity it will take me the better part of half a day to calibrate the loop. If I prime my weak and strong wash with methanol I can calibrate t0n he first or second try but when I switch back to my normal weak wash (3% ACN in water) I cannot calibrate the loop. I ran every leak and pressure check I can and the system passes. I calibrated my bubble sensor as well and it passes yet I still cannot calibrate the 2ul loop. Anything larger then 2ul presents no problem. Has anybody else experienced this problem and if so have you found a way to correct it?