Can Phosphate buffer (25mM, pH 2.6) and AcN mix on line?
<p><span>We try to convert the same HPLC method to UPLC method. It appeared mixing online gave the better resolution for 6 impurities in 4 mins. If we premixed the mobile phase (phosphate buffer pH 2.6 and AcN 75%:25%, isocratic), we have to extend our run to 4.5 min at least. Is that possible we mix phosphate buffer and AcN on line? I haven't had problem during the method development when I take caucious cleaning the buffer line. </span></p>