API analysis on UPLC
Hello UPLC Community,I am thinking about a method transfer for an active pharmaceutical ingredient (API) from a 1100 HPLC to UPLC. The HPLC method runs (~30mins, isocratic) on a classical ODS Hypersil column (250x4). The UV chromatogram shows many related compounds (known and unknown) and I expect them to shift in retention time when changing to the new BEH C18 material.Has anybody here done such a critical transfer? What are your experiences?