auto exposure time is too high - PDA warning
During an isocratic analysis using a PDA detector in 2D mode (215nm, Resolution = 3.6nm, Exposure Time = Auto), I recieved a warning that "The auto exposure time is too high". The corresponding chromatograms displayed increased area counts (6 to 25%) and/or a vertical baseline shift. Resetting the PDA detector through the Acquity Console and cycling the power on the detector did not correct the problem as the warnings continued. I converted the method to 3D mode (210 - 220nm, Resolution = 1.2nm, Exposure Time = Auto) and successfully ran my samples with no further warnings or errors. Have others had issue with the auto exposure time (and how to reset it) or collecting 2D chromatograms with a PDA detector? Any thoughts or suggestions will be greatly appreciated.