I'm interested in knowing what disadvantage there is to reducing flowrates. Common wisdom says that linear velocity in HPLC is "better" (minimum in vanDeemter curve) for large analytes (peptides/proteins) if it's low; and for small molecules, if it's high. Since UHPLC has such broad minimums in the vanDeemter curve, what disadvantage(s) is/are there to using say, 0.2 mL min for a small-molecule substance? Small molecules are less sensitive to tiny changes in gradient composition (a greater risk with low flowrates), and after all, one of the biggest advantages of UHPLC is its low solvent consumption. Just curious.