Tailing after transfer to UPLC
Hello Forum,we have transfered an isocratic method for the measurement of S-adenosylmethionin from a Waters 2795/Quattro Micro to our UPLC/Premier. The column we use on both systems is a 100x2.1mm (3.5um) Symmetry Shield with the same type guard column. The HPLC method was transfered 1:1 since we arent using a "UPLC" column. MS parameters were slightly adapted to the Premier. However, on UPLC/Premier we get a clear peak tailing where there was none on the 2795/Quattro Micro System.Any ideas were or how the tailing could be caused?