Column Temperature

BerkshireLab
BerkshireLab
in Instrumentation and Mass Spectrometry
<p>Hello, I am currently using an HSS C18 1.8 um column (2.1 mm x 150 mm). I was wondering what the recommended column temperature range is for this column. I am developing an opiate method and am testing out different temperatures. I've gone up to 60C, but wanted to check what maximum temperature is recommended for this column in order to preserve/maintain the column life and efficiency.</p><p style="height: 8pt"/><p>Thanks!</p><p>~ Kate Dedaj</p>

Answers

  • rune
    rune

    As stated in our Care and Use Manual (attached) for the HSS columns we recommend that you stay between 20-45 C for this column. Going to higher temperatures may shorten column lifetime.

  • BerkshireLab
    BerkshireLab

    Thank you so much! Also, thank you for the manual. I couldn't seem to find a manual anywhere, so that will be very helpful

  • dougm
    dougm

    All of the Care & Use manuals for our LC columns are located here.

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