Use of Column Mananger and Solvent Selector Valve to enhance Acquity method development
Background:After reading about the use of a Solvent Selector Valve to expand the rather limited number of lines on the Acquity, I was quite excited to purchase one and connect it to our newest Acquity system that was equipped with a Column Manager. I wanted to assess the feasibility of using the Solvent Selector Valve in combination with the Column Manager for broad automated method screening with four columns, two organic modifiers and six solution at various pH. I then planned on using data mining techniques championed by Waters such as total peaks and total resolution to rapidly assess the data and rank the methods. However, after several months of valiant effort I was unable to get the Solvent Selector Valve to work. I would wire the system as described by Waters but the valve would refuse to consistently switch between lines. I replaced the selector, twice. Finally our Waters Service Engineer informed me that there was an ICOP incompatibility between the Sample Manager and the Solvent Selector Valve. I was told that a bug had been identified and would be addressed in a subsequent ICOP revision but until then I would not be able to use the Solvent Selector Valve with our Acquity. Then, to my chagrin, while viewing recently the Web Seminars on the Online Acquity, I noticed another reference to the use of the Solvent Selector to enhance Acquity method development. Question:Has anyone successfully configured a Solvent Selector Valve with an Acquity Sample Manager (ICOP Firmware Version 1.30)?