I would like to create a custom field to calculate the assay of two API in the same analysis, can someone help me?
if I write you the formula, can you help me create a formula step by step?
You need to use the component editor to enter you standard amounts. You need to use the sample weights and dilutions field within the sample set method to accommodate your standard weight and dilutions.
Empower will then calculate the concentration of your analytes in the amount field.
To average the two replicates you can most easily generate an average by using the same sample label in the sample set for replicate one and two. You can then setup a report to group samples by label and give you an average for each group.
If you want to use the average value for a calculation down stream, then you will need the following custom field:
Can you describe me step by step?
In sample set, add a "Clear Calibration" line before the Std1 line. Label the 6 injections of Std1 as S0101, label the 2 injections of Std2 as S0102. Label the Samples 1 and 2 as U1 U2 U3 and U4. After the std2 line, insert a Clear Calibration, then Calibrate (making sure the label Reference on the Calibrate line is set to S01* then Quantitate with the Label Reference on Quantitate set to U*). In the component editor of your sample set make sure you have the amounts or concentrations entered in the Value tab for all standard components. Save all changes, bring the standard/sample channels into review, open processing method, and for the standard injections press integrate then calibrate, which builds your calibration curve. File>Save as Calibration to save curves. Then for your sample injections press integrate then quantitate and your amounts will appear.
Or you can batch process a sample set to generate a result set. Right click sample set, process, use specified processing method, select the method, make sure clear calibration is ticked then click ok- your result set will be generated with amounts stored. Make sure to account for any dilution factors or sampleweights for your samples before saving sample set.