Peak Tailing - Machine Related??????
<p>Hi,</p><p></p><p>I'm currently developing a method for a small molecule.</p><p></p><p>Started development on our i-class (FTN) and almost immediatley got a good looking peak. Brought the run time down to 1.2 mins. Peak tailing was around 1.3 which I was very happy about it.</p><p></p><p>Due to some scheduling issues I moved the semi-developed method onto our other i-class system. system exactley the same as the previous one. Same column and mobile phase. Tail factor now at 1.9!!</p><p></p><p>Can't figure out what is causing this change??</p><p></p><p>Looked at fittings. they seem fine. Made sure the purge and wash solutions are all correct and primed as well.</p><p></p><p>Could there be a sytem related issue causing this?</p><p></p><p>I moved the method back to the original machine and peak shape is back to how it was.</p>