Peak Tailing - Machine Related??????
Hi,I'm currently developing a method for a small molecule.Started development on our i-class (FTN) and almost immediatley got a good looking peak. Brought the run time down to 1.2 mins. Peak tailing was around 1.3 which I was very happy about it.Due to some scheduling issues I moved the semi-developed method onto our other i-class system. system exactley the same as the previous one. Same column and mobile phase. Tail factor now at 1.9!!Can't figure out what is causing this change??Looked at fittings. they seem fine. Made sure the purge and wash solutions are all correct and primed as well.Could there be a sytem related issue causing this?I moved the method back to the original machine and peak shape is back to how it was.