How to choose the best wavelength choice for procedures?
I have a question regarding the best approach to determine the proper wavelength to use in a procedure. Do I blindly follow the the literature sources for the selection of a wavelength or should I review the PDA wavelength and work with the absorbance maxima of the compound of interest? Sometimes the literature provides a wavelength selection not even close to the wavelength maxima of the compound. Why would someone choose a wavelength other than the maxima?Thanks