Sample Preparation - Sep Columns
I am preparing methods for the analysis of some very complex matrixes (Dietary supplements). Because my company is a contract manufacturer the number of products we have is immense and so are the matrixes. So far my sample prep has been very basic, weight in dilution flask and then filtered with 0.2um filter and then injected. In terms of getting accurate results, this has worked in most situations. However I really don't know what is happening with the contents of the matrix because they are so complex. My question is how do I know when more involved sample preparation is necessary either with regards to poor results or system care (sample crash out, clogging column etc etc)?