Can you confirm which version of ACQUITY you are using? Is it standard ACQUITY or ACQUITY H-Class?
Having said that, Auto-addition is available on the H-Class with Empower 3. However, we define auto-addition as sipping multiple samples from different vials and injecting them at the same time onto the column (sort of a train car approach). I believe what you desire is what we refer to as auto-transfer - the ability to add something (i.e.: reagents) to a sample vial and inject from that vial. If so, then be advised that we are working on this feature to be released in a future release of software.
It is the standard Acquity. And basically what I want to do is inject 5uL each from 2 separate vials.
Thanks for the clarification. Unlike the H-Class injector, the Fixed Loop injector that is on the standard ACQUITY does not currently have the auto-addition feature. We are looking into this as a feature enhancement for a future release.